В статье рассмотрены и обсуждены работы, посвящённые выделению фосфолипазы Д из различных источников и подробному исследованию её активации на границе раздела фаз при гидролизе лецитина и лизолецитина в присутствии ионов металлов, органических растворителей, детергентов, адсорбентов и продуктов гидролиза, а также в составе мембран митохондрий и микросом в различных условиях проведения реакции. На основании полученных результатов выдвинута гипотеза об активации, функционировании, регуляции и локализации фосфолипазы Д на границе раздела фаз липид – вода, липид – липид и липид – белок при образовании кластера, рафта или микродомена из продуктов гидролиза или их природных и синтетических аналогов в присутствии ионов двухвалентных металлов. Фосфолипаза Д рассматривается как примембранный векторный фермент, латеральный насос или как 2D-принтер, преобразующий субстрат в продукт с одновременным его депонированием с ионами Ca2+ в разрастающийся новый фосфатидатный микродомен. Обсуждены возможные приложения предложенного механизма регуляции липолиза в решении ряда вопросов и проблем мембранологии, биотехнологии, питания и медицины.
В статье рассмотрены и обсуждены работы, посвящённые выделению фосфолипазы Д из различных источников и подробному исследованию её активации на границе раздела фаз при гидролизе лецитина и лизолецитина в присутствии ионов металлов, органических растворителей, детергентов, адсорбентов и продуктов гидролиза, а также в составе мембран митохондрий и микросом в различных условиях проведения реакции. На основании полученных результатов выдвинута гипотеза об активации, функционировании, регуляции и локализации фосфолипазы Д на границе раздела фаз липид – вода, липид – липид и липид – белок при образовании кластера, рафта или микродомена из продуктов гидролиза или их природных и синтетических аналогов в присутствии ионов двухвалентных металлов. Фосфолипаза Д рассматривается как примембранный векторный фермент, латеральный насос или как 2D-принтер, преобразующий субстрат в продукт с одновременным его депонированием с ионами Ca2+ в разрастающийся новый фосфатидатный микродомен. Обсуждены возможные приложения предложенного механизма регуляции липолиза в решении ряда вопросов и проблем мембранологии, биотехнологии, питания и медицины.
Мазкур мақолада фосфолипаза Д ни турли тирик организмлардан ажратиб олишга доир тадқиқотлар, лецитин ва лизолецитин, металл ионлари, органик эритувчилар, детергентлар, адсорбентлар, гидролиз маҳсулотлари ёки уларнинг табиий ва синтетик аналоглари иштирокида ва турли реакция шароитида бу ферментнинг фаоллашуви батафсил ўрганилган ва муҳокама қилинган. Олинган натижалар асосида гидролиз маҳсулотларидан кластер, рафт ёки микродомен ҳосил бўлиши пайтида липид – сув, липид – липид ва липид – оқсил фазалари чегарасида фосфолипаза Д нинг фаоллашиши, ишлаши, регуляцияси ва локализацияси тўғрисида янги гипотеза таклиф қилинган. Фосфолипаза Д га сирт мембранали вектор ферменти, латерал насоси ёки 2D принтери сифатида қаралади. Фосфолипаза Д фосфолипид субстрати Cа2+ ионлари билан бир вақтда ўсиб бораётган микродоменда янги фосфатид маҳсулотига айланади. Шунингдек, мақолада мембранология, биотехнология, овқатланиш ва тиббиётнинг бир қатор масалалари ва муаммоларини ҳал қилишда липолизни тартибга солиш механизмининг мумкин бўлган қўлланилиши муҳокама қилинган.
The review elucidates works on isolation of phospholipase D from various sources, a detailed study of its activation at the interface during hydrolysis of lecithin and lysolecithin in the presence of metal ions, organic solvents, detergents, adsorbents and products of hydrolysis, as well as in composition of mitochondrial membranes and microsomes under various reaction conditions. Based on the research findings, a hypothesis is being put forward about activation, functioning, regulation and localization of phospholipase D at the interface of lipid-water, lipidlipid and lipid-protein phases in the course of forming of a cluster, raft or microdomain with products of hydrolysis or their natural and synthetic analogues in the presence of bivalent metal ions. Phospholipase D is considered as a pri-membrane vector enzyme, a lateral pump, or as a 2D printer that converts a substrate into a product while depositing it with Ca2+ ions into a growing new phosphatidate microdomain. Possible applications of the proposed mechanism of lipolysis regulation in solving various issues of membranology, biotechnology, nutrition and medical practice are being discussed.
| № | Muallifning F.I.Sh. | Lavozimi | Tashkilot nomi |
|---|---|---|---|
| 1 | Madyarov S.R. | biologiya fanlar doktori, yetakchi ilmiy xodim | O‘zbekiston Respublikasi Fanlar Akademiyasi Bioorganik kimyo instituti, Mirzo Ulug‘bek nomidagi O‘zbekiston milliy universiteti Biofizika va biokimyo instituti |
| № | Havola nomi |
|---|---|
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