Today, in the world agriculture, especially cotton growing sphere plays an important role in the national economy. In the cultivation of cotton one of the main problems in the fields is weeds.When estimating the damage caused by weeds to cotton fields, it accounted for 75 bln. USD. This scientific article presents data on the effectiveness of pre-and post-application of herbicides against weeds in the areas where the tillage was done with double-toothed plow in the fall before preparing beds in the fine-fiber cotton fields under typical gray soils condition.Effective eradication of annual and perennial weeds (86,8-90,2%) was observed, when a single herbicide Ankosar (3,0,4,0, 5,0 l/ha) was applied (information of 2017-2019). When the herbicides Step 500 (4,0 l/ha) and Ankosar (4,0 l/ha) were applied consecutively,the effect on annual weeds was noted (90,5-93,0%). Perennial weeds are reduced by 88,9-93,3% when the abovementioned preparationsare applied one after another. Cotton yield will increase by 1,8-5,4 c/ha compared to the control variant. It is recommended to apply the herbicides consecutively in the order Ankosar (4,0 l/ha), Step 500 (4 l/ha) and Ankosar (4.0 l/ha) against annual and perennial weeds.