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ОПТИМИЗАЦИЯ МЕТОДОВ ВЫДЕЛЕНИЯ И ОЧИСТКИ ДНК ДЛЯ МОЛЕКУЛЯРНОГЕНЕТИЧЕСКОГО АНАЛИЗА НЕКУЛЬТИВИРУЕМЫХ МИКРООРГАНИЗМОВ РУБЦА КОРОВ

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MAQOLA ANNOTATSIYASI

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Мақолада клиник жиҳатдан соғлом сигирларда жароҳат микроорганизмларининг жами ДНКларини ажратиб олиш ва тозалаш усулларини оптималлаштириш кўрсатилган.

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Teglar

# рубец# cattle# микробиота# молекуляр-генетик анализ# ямоқ# чорва ҳайвонлари# молекулярно-генетический анализ# крупный рогатый скот# microbiota# molecular-genetic analysis# rumen

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Maqola idintifikatorlari

Foydalanilgan adabiyotlar

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2. Hungate R.E. A roll tube method for cultivation of strict anaerobes. In: Norris J.R., Ribbons D.W. (eds). Methods in microbiology, vol. 3B. Academic Press, London and New York, p. 117–132, 1969.

3. Dehority B.A, Tirabasso P.A, Grifo AP. Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Appl. Environ. Microbiol. 55:2789– 2792, 1989.

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7. Forster R., J. Gong, and R. Teather. Group-specific 16S rRNA hybridization probes for determinative and community structure studies of Butyrivibrio fibrisolvens in the rumen // Appl. Environ. Microbiol. - 1997. – Vol.63.- P.1256- 1260.

8. Whitehead T.R. and M.A. Cotta. Characterization and comparison of microbial populations in swine faeces and manure storage pits by 16S rDNA gene sequence analyses // Anaerobe. - 2001. – Vol.7. -P.181-187

9. Yu Z. and M. Morrison. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis // Appl. Environ. Microbiol. - 2004. – Vol.70. №8.- P.4800-4806.

10. Flint H.J., A.M. Thomson. Deoxyribonuclease activity in isolated rumen bacteria and rumen fluid // Lett. Appl. Microbiol. - 1990. – Vol.11. -P.18-21.

11. Tebbe C. C. and W. Vahjen. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast // Appl. Environ. Microbiol. - 1993. – Vol.59. -P.2657-2665.

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