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PICHIA PASTORIS АЧИТҚИ ЭКСПРЕССИЯ ТИЗИМИДА РЕКОМБИНАНТ ОҚСИЛЛАР ОЛИШ УЧУН МАҚБУЛ ШТАММ ВА ТРАНСФЕР ВЕКТОРЛАРНИ ТАНЛАШ

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MAQOLA ANNOTATSIYASI

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Мақола ачитқи эскпрессия тизимида биологик фаол рекомбинант оқсиллар олиш учун Pichia pastoris ачитқиси ва трансфер векторлардан фойдаланишга қаратилган. Таҳлил натижаларига кўра GS 115 Pichia pastoris штамми ва унга ёт генларни самарали интеграция қилиш имкониятини берадиган pPIC3,5 ва pPIC9 трансфер векторлари танлаб олинган.

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# Рекомбинант оқсиллар# ачитқи экспрессия тизими# Pichia pastoris# GS 115 штамм# трансфер векторлари# Рекомбинантные белки# дрожжевая система экспрессии# штамм GS 115# трансферные векторы# Recombinant proteins# yeast expression system# strain GS 115# transfer vectors

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Foydalanilgan adabiyotlar

1) Ahmad M., Hirz M., Pichler H., Schwab H. Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production // Appl Microbiol Biotechnol. 2014, 98:5301–5317

2) Vogl T, Hartner FS, Glieder A. New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris // Current Opinion in Biotechnology. 2013, 24:1094–1101.

3) http://www.pichia.com/science-center/commercialized-products/

4) Gasser B, Prielhofer R, Marx H, Maurer M, Nocon J, Steiger M, Puxbaum V, Sauer M, Mattanovich D. Pichia pastoris: protein production host and model organism for biomedical research // Future Microbiol. 2013, 8:191-208.

5) Schutter K.D., Lin Y.-Ch., Tiels P., Hecke A.V., Glinka S., Weber-Lehmann J., Rouze P., Peer V.Y., Callewaert N. Genome sequence of the recombinant protein production host Pichia pastoris // Nature Biotechnology. 2009, vol. 27, 6:561-566

6) Hamilton S.R. et al. Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins // Science 2006, 313, 1441-1443.

7) Hamilton S.R., Gerngross T.U. Glycosylation engineering in yeast: the advent of fully humanized yeast // Curr. Opin. Biotechnol. 2007, 18, 387-392.

8) Jacobs P.P., Geysens S., Vervecken W., Contreras R., Callewaert N. Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology // Nat. Protoc. 2009, 4, 58-70

9) Hartner F.S. et al. Promoter library designed for fne-tuned gene expression in Pichia pastoris. // Nucleic Acids Res. 2008, 36, e76.

10) Cregg J.M., Cereghino J.L., Shi J., Higgins D.R. Recombinant protein expression in Pichia pastoris // Molecular Biotechnology. 2000, 16, 23-52.

11) Pichia Expression Kit for expression of recombinant proteins in Pichia pastoris. User Guide. Publication Number MAN0000012, 2014.

12) Green M.R., Sambrook J. // Molecular cloning. A laboratory manual. Fourth edition. New York: Cold Spring Harbor Laboratory Press, 2012.

13) https://www.ncbi.nlm.nih.gov/

14) https://www.snapgene.com/academics/for-academics/

15) http://ugene.net/ru/.

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