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COMPOSITION OF ESSENTIAL OIL FROM DRIED BASIL (Ocimum Basilicum L.)

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The present work describes a methodological approach for the isolation and analysis of essential oils (EOs) obtained from finely ground basil plants. The extraction of basil EO was carried out by hydrodistillation using a classical Clevenger apparatus for a duration of three hours. The distillates were subsequently treated with n-hexane, followed by dehydration with anhydrous sodium sulfate in order to remove residual moisture. The yield of the essential oil under these conditions varied within the range of 0.74–0.81%. The isolated oils were characterized as pale-yellow, mobile liquids possessing a specific aromatic odor typical of basil. For the identification of chemical constituents, the qualitative and quantitative composition of the oils was studied using gas chromatography–mass spectrometry (GC–MS). The analyses were performed on an Agilent 5975C inert MSD/7890A GC instrument equipped with a quartz capillary column Agilent HP-INNOWax, under a programmed temperature regime. This integrated approach enables reliable isolation, precise characterization, and effective identification of the component profile of basil essential oils.

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# yield# component composition# Hydrodistillation# basil# ssential oils

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5. Mambetsheripova, A. A., Sultanova, Sh. A., Safarov, J. E. Changes in the properties of food materials of plant origin during drying at the cellular level. In: Innovative Solutions: Sustainable Development in Agriculture and Food Industry: Proc. of the V Int. Sci. and Tech. Conf. Tashkent, 2025, pp. 278–279. (in Russian) 6. Mambetsheripova, A. A., Makhmudov, K. A., Safarov, J. E., Sultanova, Sh. A. Study of basil (Ocimum basilicum L.) as an object for drying. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the Int. Sci. and Pract. Conf. Kiev, 2024, pp. 147–150.

1. Tkachev, A. V. Investigation of Volatile Substances of Plants. Novosibirsk: Ofset Publ., 2008. 969 p. (in Russian) 2. Avrămia, I., Oroian, M.-A., Oiţă, R.-C. A review of current trends of vitamin identification and quantification by chromatography from food samples. Journal of Food Composition and Analysis, 2024, vol. 131, art. 106244. 3. Amithabh, G. S., Kumar, T., Gireesh Kumar, M. P., Kaviya, S., Baskar, B. Development and validation of a novel HPLC-UV method for quantifying vitamin D forms and precursors in vegetable oils after exposure to sunlight and UV radiation. Acta Chromatographica, 2024. https://doi.org/10.1556/1326.2024.01275

4. Sultanova, Sh. A., Safarov, J. E., Usenov, A. B., Mambetsheripova, A. A. Analysis of essential oil extraction technology from basil plant. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the XIII Int. Sci. and Pract. Conf. Kiev, 2025, pp. 28–30. (in Russian)

7. Mambetsheripova, A. A., Safarov, J. E., Sultanova, Sh. A. Changes in the properties of food materials of plant origin during drying at the cellular level. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the Int. Sci. and Pract. Conf. Kiev, 2024, pp. 131–133. 8. Sultanova, Sh. A., Mambetsheripova, A. A., Safarov, J. E. Energy-efficient drying method. In: Actual Problems of Power Supply Systems: Proc. of the III Int. Sci. and Tech. Conf. Tashkent, 2023, pp. 54–55. (in Russian) 9. Sultonova, S.A., Saparov, J.E., Mambetsheripova, A. A., Usenov, A. B., Alimova, D. Q. Control of modes of energy-saving vibro-drying devices. E3S Web of Conferences, 2023, vol. 461, art. 01059, pp. 1-4.

1. Tkachev, A. V. Investigation of Volatile Substances of Plants. Novosibirsk: Ofset Publ., 2008. 969 p. (in Russian) 2. Avrămia, I., Oroian, M.-A., Oiţă, R.-C. A review of current trends of vitamin identification and quantification by chromatography from food samples. Journal of Food Composition and Analysis, 2024, vol. 131, art. 106244. 3. Amithabh, G. S., Kumar, T., Gireesh Kumar, M. P., Kaviya, S., Baskar, B. Development and validation of a novel HPLC-UV method for quantifying vitamin D forms and precursors in vegetable oils after exposure to sunlight and UV radiation. Acta Chromatographica, 2024. https://doi.org/10.1556/1326.2024.01275

4. Sultanova, Sh. A., Safarov, J. E., Usenov, A. B., Mambetsheripova, A. A. Analysis of essential oil extraction technology from basil plant. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the XIII Int. Sci. and Pract. Conf. Kiev, 2025, pp. 28–30. (in Russian)

5. Mambetsheripova, A. A., Sultanova, Sh. A., Safarov, J. E. Changes in the properties of food materials of plant origin during drying at the cellular level. In: Innovative Solutions: Sustainable Development in Agriculture and Food Industry: Proc. of the V Int. Sci. and Tech. Conf. Tashkent, 2025, pp. 278–279. (in Russian) 6. Mambetsheripova, A. A., Makhmudov, K. A., Safarov, J. E., Sultanova, Sh. A. Study of basil (Ocimum basilicum L.) as an object for drying. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the Int. Sci. and Pract. Conf. Kiev, 2024, pp. 147–150.

7. Mambetsheripova, A. A., Safarov, J. E., Sultanova, Sh. A. Changes in the properties of food materials of plant origin during drying at the cellular level. In: Scientific Achievements in Solving Current Problems of Production and Processing of Raw Materials, Standardization and Food Safety: Proc. of the Int. Sci. and Pract. Conf. Kiev, 2024, pp. 131–133. 8. Sultanova, Sh. A., Mambetsheripova, A. A., Safarov, J. E. Energy-efficient drying method. In: Actual Problems of Power Supply Systems: Proc. of the III Int. Sci. and Tech. Conf. Tashkent, 2023, pp. 54–55. (in Russian) 9. Sultonova, S.A., Saparov, J.E., Mambetsheripova, A. A., Usenov, A. B., Alimova, D. Q. Control of modes of energy-saving vibro-drying devices. E3S Web of Conferences, 2023, vol. 461, art. 01059, pp. 1-4.

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