INVESTIGATION OF ELECTROSTATIC EFFECTS IN SIZE EXCLUSION CHROMATOGRAPHY OF POLYSACCHARIDES

Size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups and neutral fractions of larch arabinogalactans (AG) were separated from neutral molecules in various concentrations of the injected samples. Electrostatic effects in separation of carboxymethylcelluloses (CMC) were demonstrated by concentration dependence of retention volume at various injected sample concentrations.