Background: Helicobacter pylori (H. pylori) remains one of the most common human infections in Sudan recently and is associated with a number of important chronic gastritis, peptic ulcer disease and gastric malignancy. Objectives: The aim of this study was to compare the detection of H. pylori IgG in serum using ELISA techniques compared to ICT blood test and stool antigen. Materials and Methods: Stool and blood specimens were collected from 100 patients (mean age 31.2 ± 11.7 years, 56% males). Stool samples were analyzed using rapid stool antigen test for H. pylori and Serum samples were analyzed for H. pylori IgG by Accurate© (USA) ELISA and ICT blood test. Data analysis was made by the software of the Statistical Package for Social Sciences (SPSS) program (version 22). Results: The incidence of H. pylori among male was 12/17 (71%) compare to 5/17 (29%) females. 17 (17%) patients have positive with rapidstoolAg ICT test compare to 20 (20%) patients have positive by H. pylori IgG ELISA [the Accurate© (USA)]. The sensitivity, specificity, positive predictive value and negative predictive value for H. pylori IgG ELISA were (100%, 96.1%, 93.3% and 100% respectively) compared to ICTH. pylori IgG for blood (41.18%, 71.08%, 43.4% and 69.2% respectively) using rapid stool Ag ICT test as gold standard method. Conclusion: The better results Sensitivity and Specificity obtained for H. Pylori diagnosis wasH. pylori IgG using ELISA techniques compared to ICT blood test.
Background: Helicobacter pylori (H. pylori) remains one of the most common human infections in Sudan recently and is associated with a number of important chronic gastritis, peptic ulcer disease and gastric malignancy. Objectives: The aim of this study was to compare the detection of H. pylori IgG in serum using ELISA techniques compared to ICT blood test and stool antigen. Materials and Methods: Stool and blood specimens were collected from 100 patients (mean age 31.2 ± 11.7 years, 56% males). Stool samples were analyzed using rapid stool antigen test for H. pylori and Serum samples were analyzed for H. pylori IgG by Accurate© (USA) ELISA and ICT blood test. Data analysis was made by the software of the Statistical Package for Social Sciences (SPSS) program (version 22). Results: The incidence of H. pylori among male was 12/17 (71%) compare to 5/17 (29%) females. 17 (17%) patients have positive with rapidstoolAg ICT test compare to 20 (20%) patients have positive by H. pylori IgG ELISA [the Accurate© (USA)]. The sensitivity, specificity, positive predictive value and negative predictive value for H. pylori IgG ELISA were (100%, 96.1%, 93.3% and 100% respectively) compared to ICTH. pylori IgG for blood (41.18%, 71.08%, 43.4% and 69.2% respectively) using rapid stool Ag ICT test as gold standard method. Conclusion: The better results Sensitivity and Specificity obtained for H. Pylori diagnosis wasH. pylori IgG using ELISA techniques compared to ICT blood test.
| № | Author name | position | Name of organisation |
|---|---|---|---|
| 1 | Mohammed S.A. | student | Ministry of Health Gezira State, Sudan |
| № | Name of reference |
|---|---|
| 1 | 1- Chey, W.D. and Wong, B.C., (2007). American College of Gastroenterology guideline on the management of Helicobacter pylori infection. The American journal of gastroenterology, 102(8), p.1808. |
| 2 | 2- Brooks, H.J.L., Ahmed, D., McConnell, M.A. and Barbezat, G.O., (2004). Diagnosis of Helicobacter pylori infection by polymerase chain reaction: is it worth it?. Diagnostic microbiology and infectious disease, 50(1), pp.1-5. |
| 3 | 3- Shamsuddeen, U., Yusha’u, M. and Adamu, I.A., (2009). Helicobacter pylori: the causative agent of peptic ulcer.Bayero Journal of Pure and Applied Sciences, 2(2), pp.79-83. |
| 4 | 4- Everhart, J.E., Kruszon-Moran, D., Perez-Perez, G.I., Tralka, T.S. and McQuillan, G., (2000). Seroprevalence and ethnic differences in Helicobacter pylori infection among adults in the United States. The Journal of infectious diseases, 181(4),pp.1359- 1363. |
| 5 | 5- Kabir, S.,( 2001). Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. Journal of medical microbiology, 50(12), pp.1021- 1029. |