The widespread use of the stimulant drug 3,4-methylenedioxymethamphetamine (MDMA), commonly referred to as Ecstasy, Molly, or X, has been associated with life-threatening hyperthermia in both humans and animal models. This study aimed to explore the role of the gut-adrenal axis in MDMA-induced hyperthermia by examining the effects of acute exogenous supplementation with norepinephrine (NE) or corticosterone (CORT) in adrenalectomized (ADX) rats following MDMA administration. MDMA (10 mg/kg, subcutaneous) significantly elevated body temperature in SHAM animals compared to ADX animals at 30-, 60-, and 90-minute intervals post-treatment. The attenuated hyperthermic response observed in ADX animals was partially restored with exogenous administration of NE (3 mg/kg, intraperitoneal) or CORT (3 mg/kg, intraperitoneal) 30 minutes after MDMA treatment. Additionally, 16S rRNA analysis revealed notable differences in gut microbiome composition and diversity, including a higher abundance of minor phyla Actinobacteria, Verrucomicrobia, and Proteobacteria in ADX rats compared to control andSHAM rats. MDMA administration further induced significant changes in the dominant phyla Firmicutes and Bacteroidetes, as well as minor phyla Actinobacteria, Verrucomicrobia, and Proteobacteria in ADX animals. Exogenous CORT treatment increased Bacteroidetes and decreased Firmicutes, while NE treatment increased Firmicutes and decreased Bacteroidetes and Proteobacteria. These findings highlight a potential relationship between the sympathoadrenal axis, gut microbiome composition, and MDMA-induced hyperthermia
The widespread use of the stimulant drug 3,4-methylenedioxymethamphetamine (MDMA), commonly referred to as Ecstasy, Molly, or X, has been associated with life-threatening hyperthermia in both humans and animal models. This study aimed to explore the role of the gut-adrenal axis in MDMA-induced hyperthermia by examining the effects of acute exogenous supplementation with norepinephrine (NE) or corticosterone (CORT) in adrenalectomized (ADX) rats following MDMA administration. MDMA (10 mg/kg, subcutaneous) significantly elevated body temperature in SHAM animals compared to ADX animals at 30-, 60-, and 90-minute intervals post-treatment. The attenuated hyperthermic response observed in ADX animals was partially restored with exogenous administration of NE (3 mg/kg, intraperitoneal) or CORT (3 mg/kg, intraperitoneal) 30 minutes after MDMA treatment. Additionally, 16S rRNA analysis revealed notable differences in gut microbiome composition and diversity, including a higher abundance of minor phyla Actinobacteria, Verrucomicrobia, and Proteobacteria in ADX rats compared to control andSHAM rats. MDMA administration further induced significant changes in the dominant phyla Firmicutes and Bacteroidetes, as well as minor phyla Actinobacteria, Verrucomicrobia, and Proteobacteria in ADX animals. Exogenous CORT treatment increased Bacteroidetes and decreased Firmicutes, while NE treatment increased Firmicutes and decreased Bacteroidetes and Proteobacteria. These findings highlight a potential relationship between the sympathoadrenal axis, gut microbiome composition, and MDMA-induced hyperthermia
| № | Муаллифнинг исми | Лавозими | Ташкилот номи |
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| 1 | Usanova S.T. | Senior lecturer | Alfraganus university |
| № | Ҳавола номи |
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