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This article studies feasibilities of making an implant-film for treatment of burn wound, based on silver nanoparticles (AgNPs) stabilized by sodiumcarboxymethylcellulose (Na-CMC) with degrees of substitution (DS) 0.65-0.90 and polymerization (DP) 200-600 synthesized using a photochemical method. The structural, physical and chemical, and physicalmechanical, burn wound healing properties as well as antimicrobial effects of implant-films containing the Na-CMC and AgNPs have been investigated. The shape, quantity, and size of the AgNPs embedded into the Na-CMC films were determined by UV-Vis spectroscopy, dynamic light scattering (DLS), atomic force microscopy (AFM) and transmission electron microscopy (TEM). It was found that the increase of silver nitrate concentration in solution of Na-CMC followed by photoirradiation leads to changes of AgNPs size and shape. It was found out that Na-CMC films containing AgNPs in the size ranging from 5 to 35 nm had enhanced the microbicide effects and burn wound healing properties within 14 days.

  • Количество прочтений0
  • Дата публикации24-02-2022
  • Язык статьиIngliz
  • Страницы56-68
Ўзбек

Мазкур мақолада алмашиниш даражаси (АД) 0,65-0,85 ва полимерланиш даражаси (ПД) 200-600 бўлган натрий-карбоксиметилцеллюлоза (Na-КМЦ) эритмасида барқарор кумуш нанозарралари (АgНЗ) фотокимёвий қайтарилиш усулида синтез қилинган. Таркибида АgНЗ ва Na-КМЦ тутган плёнкаларнинг физик-кимёвий, физик-механик ва куйган яраларни даволовчи хоссалари ҳамда бактерицид фаоллиги ўрганилган. Na-КМЦ плёнкаларида шаклланган АgНЗнинг ўлчами, шакли ва миқдори атом кучланишли микроскопия (АКМ), УБ-спектроскопия, динамик ёруғлик тарқалиши (DLS) ва трансмиссион электрон микроскопия (TЭМ) усуллари орқали аниқланган. Na-КМЦ ва у асосида олинган имплант-плёнкаларда кумуш нитрат тузи концентрацияси ортиши билан фотокимёвий қайтариш натижасида шаклланаётган АgНЗ ўлчам ва шаклларининг ўзгариши аниқланди. Ўлчамлари 5–35 нм бўлган АgНЗ тутган Na-КМЦ плёнкалар юқори бактерицид фаоллик намоён қилганлиги ва 14 кун давомида куйган яраларни даволаш хусусиятига эга эканлиги аниқланди.

Русский

В данной статье стабильные наночастицы серебра (НЧAg) были синтезированы фотохимическим методом в растворе натрий-карбоксиметилцеллюлозы (Na-КМЦ) со степенью замещения (СЗ) 0,65-0,85 и степенью полимеризации (СП) 200-600. Изучены структура, физико-химические, физико-механические и противоожоговые свойства, а также бактерицидная активность пленок Na-КМЦ, содержащих НЧAg. Методами атомно-силовой микроскопии (АСМ), УФ-спектроскопии, динамического рассеяния света (DLS) и просвечивающей электронной микроскопии (ПЭМ) определены форма и размеры НЧAg, присутствующие в пленках Na-КМЦ. Установлено, что с увеличением концентрации нитрата серебра в растворах Na-КМЦ в процессе фотооблучения происходит изменение размера и формы НЧAg. Экспериментально доказано, что пленки Na-KMЦ, содержащие НЧAg размером от 5–35 нм, обладают высокой бактерицидной активностью и ранозаживляющими свойствами при ожоговых ранах в течение 14 суток.

English

This article studies feasibilities of making an implant-film for treatment of burn wound, based on silver nanoparticles (AgNPs) stabilized by sodiumcarboxymethylcellulose (Na-CMC) with degrees of substitution (DS) 0.65-0.90 and polymerization (DP) 200-600 synthesized using a photochemical method. The structural, physical and chemical, and physicalmechanical, burn wound healing properties as well as antimicrobial effects of implant-films containing the Na-CMC and AgNPs have been investigated. The shape, quantity, and size of the AgNPs embedded into the Na-CMC films were determined by UV-Vis spectroscopy, dynamic light scattering (DLS), atomic force microscopy (AFM) and transmission electron microscopy (TEM). It was found that the increase of silver nitrate concentration in solution of Na-CMC followed by photoirradiation leads to changes of AgNPs size and shape. It was found out that Na-CMC films containing AgNPs in the size ranging from 5 to 35 nm had enhanced the microbicide effects and burn wound healing properties within 14 days.

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