Melanin ajratish bo'yicha qo'llanilgan 2 ta usul solishtirilganda, 2-usulni optimal deb xisoblaymiz. Bunga sabab, 2 usulda 1 usulga nisbatan kamroq reagentlar va asbob—uskunalar talab qiladi hamda melanin ajratishga ketadigan vaqt ancha qisqaradi. Bundan tashqari,2-usulda melaninni hosil bo'lishini aniqlash sezgirligi 1-usulga nisbatan biroz bo'lsada yuqori ekanligi qayd etildi. Xar ikkala usulda xam melaninni eng ko'p sintez qiladigan A4-13 va C17-1 shtammlari ekanligi aniqlandi.Shunday qilib, dinamikada o`stirilgan stammning tajribada qo`llanilgan oziqa muhitlar o`rtasida melaninni yuqori sintez qilishi kartoshka va temir sitratli oziqa muhiti optimal ekanligi aniqlandi
Melanin ajratish bo'yicha qo'llanilgan 2 ta usul solishtirilganda, 2-usulni optimal deb xisoblaymiz. Bunga sabab, 2 usulda 1 usulga nisbatan kamroq reagentlar va asbob—uskunalar talab qiladi hamda melanin ajratishga ketadigan vaqt ancha qisqaradi. Bundan tashqari,2-usulda melaninni hosil bo'lishini aniqlash sezgirligi 1-usulga nisbatan biroz bo'lsada yuqori ekanligi qayd etildi. Xar ikkala usulda xam melaninni eng ko'p sintez qiladigan A4-13 va C17-1 shtammlari ekanligi aniqlandi.Shunday qilib, dinamikada o`stirilgan stammning tajribada qo`llanilgan oziqa muhitlar o`rtasida melaninni yuqori sintez qilishi kartoshka va temir sitratli oziqa muhiti optimal ekanligi aniqlandi
First of all, we arecomparing the 2 methods used for separating melanin, we consider the 2nd method to be optimal. Because of this, 2 methods require less reagents and equipment than 1 method, and the time required for melanin separation is much shorter. In addition, it was noted that the sensitivity of the detection of melanin formation in the 2nd method is slightly higher than that of the 1st method. In both methods, it was determined that A4-13 and C17-1 strains synthesize the most melanin
| № | Имя автора | Должность | Наименование организации |
|---|---|---|---|
| 1 | Raximova K.A. | ! | Toshkent Farmatsevtika Instituti |
| № | Название ссылки |
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| 1 | 1.Park JY, Shigenaga MK, Ames BN. Induction of cytochrome P4501A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin or indolo (3,2-b) carbazole is associated with oxidative DNA damage. Proc Natl Acad Sci U S A 1996;93:2322–7.2.Protective Effects of Tea Melanin against 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity: antioxidant Activity and Aryl Hydrocarbon Receptor Suppressive Effect. Biol Pharm Bull 2006;29:2284–91.3.Avramidis A, Kourounakis A, Hadjipetrou L, Senchuk V. Antiinflammatory and immunomodulating properties of grape melanin. Inhibitory effects on paw edema and adjuvant induced disease. Arzneimittelforschung 1998;48:764–71.4.El-Obeid A, Kamal El Din E, Abdelhalim MAK, Haseeb A. Protective action of herbal melanin against carbon tetrachloride induced Hepatotoxicity. Proc. of the Third Intl. Conf. on Advances in Applied Science and Environmental Engineering 2015;1:6.5.Kamei H, Koide T, Kojima T, Hasegawa M, UmedaT. Suppression of growth of cultured malignant cells by all melanins, plantproduced melanins. Cancer Biother Radiopharm 1997;12:47–9.6.Kamei H, Koide T, Hashimoto Y, Kojima T, Hasegawa M, Umeda T. Effect of all melanin on tumor growth suppression in vivo and on the cell cycle phase. Cancer Biother Radiopharm 1997;12:273–6.7.Offen D, Ziv I, Barzilai A, Gorodin S, Glater E, Hochman A et al. Dopamine-melanin induces apoptosis in PC12 cells; possible implications for the etiology of Parkinson’s disease. Neurochem Inter 1997;31:206–17.8.Blinova MI, Yudintseva NM, Kalmykova NV, Kuzminykh EV, Yurlova NA, Ovchinnikova OA et al. Effect of melanins from black yeast fungi on proliferation and differentiation of cultivated human keratinocytes and fibroblasts. Cell Biol Int 2003;27:135–46.9.Mimura T, Itoh S, Tsujikawa K, Nakajima H, Satake M, Kohama Y et al. Studies on biological activities of melanin from marine animals. Anti-inflammatory activity of low-molecular-weight melanoprotein from Squid (Fr. SM II) . Chem Pharm Bull 1987;35:1144–50.10.El-Obeid A, ELTahirb KH, Haseeb AM. Anti-inflammatory effects of Nigella sativa L. Melanin. World J Pharm Res 2016;5:155–61. |