DEFINITION OF THE TOXICITY OF THICK CHLORELLA EXTRACT

200

It is given the composition of the thick chlorella extract. The research showed lack of acute toxicity of
thick chlorella extract concerning P. caudatum. Chlorella extract solutions stimulated growth of number
of a test object in the frst 6 h an experiment in the test concentration. The received results can be used for
preliminary estimate of toxicity at screening studies of pharmaceutical substances.

Журнал номиФармацевтика журнали
Нашр номи№1 2019
Кўришлар сони 200

Internet ҳавола

DOI

UzSCI тизимида яратилган сана 23-11-2019

Ўқишлар сони 198

Нашр санаси 04-04-2019

Мақола тилиIngliz

Саҳифалар сони98-102

Калит сўзлар

toxicity

biotesting

infusorium

thick chlorella extract

English

It is given the composition of the thick chlorella extract. The research showed lack of acute toxicity of
thick chlorella extract concerning P. caudatum. Chlorella extract solutions stimulated growth of number
of a test object in the frst 6 h an experiment in the test concentration. The received results can be used for
preliminary estimate of toxicity at screening studies of pharmaceutical substances.

Калит сўзлар

toxicity

biotesting

infusorium

thick chlorella extract

Русский

В статье приведен состав экстракта хлореллы густого. Проведенное исследование показало
отсутствие острой токсичности экстракта хлореллы густого в отношении P. caudatum.
Растворы экстракта хлореллы стимулировали рост количества клеток испытуемого объекта за
первые 6 часов эксперимента по сравнению с контрольной концентрацией.
Полученные результаты могут быть использованы для прогнозирования и предварительной
оценки токсичности при скрининговых исследованиях фармацевтических субстанций, ароматических и пищевых продуктов.

Калит сўзлар

токсичность

биотестирование

инфузории

экстракт хлореллы густой

Ўзбек

Maqolada xlorella quyuqekstrakti tarkibi keltirilgan. O'tkazilgan tadqiqotlar P. caudatum ga nisbatan quyuq xlorella ekstraktıning o’tkir zaxarliligi yo'qligini ko'rsatdi. Xlorella quyuq ekstraktı sinov
namunalari bilan taqqoslaganda tajribaning dastlabki 6 soati davomida hujayralar sonining o'sishini
rag'batlantirdi.
Olingan natijalar farmatsevtik moddalar, aromatic va oziq-ovqat mahsulotlarini skrining tadqiqotlaridagi zaxarlilikni prognoz baholash va dastlabki baholash uchun ishlatilishi mumkin.

Калит сўзлар

biotest

infusoria

chlorella ekstrakti

quyuq

zaxarlilik

№ Муаллифнинг исми Лавозими Ташкилот номи

№ Ҳавола номи

1 1. Salnikova M. Ya. Chlorella – a New Type of a Forage. - Moscow, Kolos,1977.pp. 51-53.

2 2. Karpov A. M., Albitskaya O. N. Development of Industrial Lines for Production and Processing of Microalgae. – M., Biotechnology,1989. 3, part 5, 376-380.

3 3. Spolaore P. 2006. Commercial Applications of Microalgae. - Journal of Bioscience and Bioengineering. 101(6), 201–211.

4 4. Roberts S.C. 2007. Production and Engineering of Terpenoids in Plant Cell Culture. - Nature Chemical Biology. 3, 387- 395.

5 5. Shpichka, A. I. Semenov E. F. The Comparative Characteristic of the Microorganisms Synthesizing De Novo Flying Fragrant Substances. - Basic Researches, 2013. 8 (5), 1113–1124.

6 6. Mitishev A. V., Presnyakova E. V., Semenova E. F., etc. Comparative Analysis of Strains of a Producer and Innovative Product as Basic Elements of Biotechnology of Chlorella Resinoid. - News of Higher Educational Institutions. Natural Sciences Series, Volga region, 2014. 4(8), 19-29

7 7. Haga N., Haneda K. 2007. Paramecium as a Bioassay System for Elucidation of Cytotoxicity and Biocompatibility of Nanoparticles: Effects of Carbon Nanofbers on Proliferation and Survival. -Jpn. J. Protozool. 40 (2), 139–146.

8 8. Vinokhodov D. O. Biotesting on Infusoria Cultures in Diagnostic Prevention of Animals Food Poisonings (review). - Veterinary Patologiya. 2006.1, 90-96.

9 9. Shadrin I. A., Vasilyeva T. V. Assessment of Toxicity of Soils of Krasnoyarsk Vicinities by Biotesting Methods. - Bulletin of the Krasnoyarsk State Agricultural University, 2015. 2, 32-38.

10 10. Ignatyev A. D., Isaev M. K., Dolgov V. A., etc. Modifcation of a Method of Biological Assessment of Foodstuff by Means of Ciliate of TetrakhimenPiriphormis. - Food Questions, 1980. - 1, 70-71.

11 11. Demidenko G. A., Shuranov V. V. The Assessment of Toxicity of Forages with Use of Infusoria of Paramecium Caudatum. - Bulletin of the Krasnoyarsk State Agricultural University. 2015, 10, 5-11.

12 12. Recommendations about Assessment of Toxicity of a Surface Water of Landin the Conditions of Emergency Situations by Biotesting Express Method: Р 52.24.741. – Rostov-on-Don, 2010.

13 13. V. A. Senkevich (Burdova), T. G. Stojko, News of Higher Educational Institutions. Natural Sciences Series, Volga region, 2015. 6(3), 84-93.

14 14. Kokova V. E., Lisovsky G. M. Disproportionate and Flowing Culture of Protozoa. - Novosibirsk, Science, 1982

15 15. Nikitina L. I., Solodovnikova M. V. Inﬂuence of Iron Salt on Paramecium Caudatum Activity. - Topical Issues of Modern Science. 2009, 6-1, 53-59.

16 16. Kulikov Yu. I., Dadyan N. K., Cheremnykh E. G. Biological Assessment of Toxicity of Food Dyes. - Bulletin of the North Caucasian Federal University. 2009, 2, 63-65.

17 17. Grozdov A. O. Defnition of the General Toxicity on Paramecium Caudatum. - Compound Feeds. 2001, 4, 31-33.

18 18. Bubnov A.G., Buymova S.A., Gushchin A. A., etc. The Biotest Analysis – an Integrated Method of Assessment of Quality of Objects of the Environment. Ivanovo, Ivan. State Chemical - Technol. University. 2007.

19 19. Kokova V. E. Continuous Cultivation of Invertebrates. - Novosibirsk, Science, 1982.

20 20. Karpukhina O. V., Gumargaliyeva K.Z., Inozemtsev A. N., etc. Features of Kinetics of Growth of ParameciumCaudatum Culture in Model of an Oxidizing Stress. - Bulletin of the Kazan Technological University, 2015. 10 (18), 9-11.

21 21. Methodical Recommendations about Application of Methods of Biotesting for Assessment of Water Quality in Systems of Economic and Drinking Water Supply: MR TsOS PV P 005-95. - M., 1995.

22 22. Turovtsev V. D., Krasnov B.C. Bioindication. - Tver, Tver State University, 2004.pp. 153.

23 23. Ampere-second 1638157 USSR. The Way of Receiving Resinoid Microalgae / Bugorsky P. S., Rodov V.S., Semenov E. F., Klyachko-Gurvich G. L. (USSR). – The application 22.03.89 (the application No. 4665003 with priority date of the invention 22.03.1989). It is registered in the State register of inventions of the USSR 01.12.1990. Publ. 30.03.91, BI No. 12.

24 24. V.V. Polevoy, G.B. Maksimov (ed.), Methods of biochemical analysis of plants, Publishing house of the Leningrad University, Leningrad (1978), pp. 90-99.

25 25. A Study Guide on Hygienic Rationing of Medicines in Air of a Working Zone, Atmospheric Air of the Inhabited Places and Water Objects: MU 1.1.726-98. – M, 1998.

26 26. Plokhinsky N. A. Mathematical Methods in Biology. - Moscow, MGU Publishing House, 1978.

Кутилмоқда

№	Ҳавола номи
1	1. Salnikova M. Ya. Chlorella – a New Type of a Forage. - Moscow, Kolos,1977.pp. 51-53.
2	2. Karpov A. M., Albitskaya O. N. Development of Industrial Lines for Production and Processing of Microalgae. – M., Biotechnology,1989. 3, part 5, 376-380.
3	3. Spolaore P. 2006. Commercial Applications of Microalgae. - Journal of Bioscience and Bioengineering. 101(6), 201–211.
4	4. Roberts S.C. 2007. Production and Engineering of Terpenoids in Plant Cell Culture. - Nature Chemical Biology. 3, 387- 395.
5	5. Shpichka, A. I. Semenov E. F. The Comparative Characteristic of the Microorganisms Synthesizing De Novo Flying Fragrant Substances. - Basic Researches, 2013. 8 (5), 1113–1124.
6	6. Mitishev A. V., Presnyakova E. V., Semenova E. F., etc. Comparative Analysis of Strains of a Producer and Innovative Product as Basic Elements of Biotechnology of Chlorella Resinoid. - News of Higher Educational Institutions. Natural Sciences Series, Volga region, 2014. 4(8), 19-29
7	7. Haga N., Haneda K. 2007. Paramecium as a Bioassay System for Elucidation of Cytotoxicity and Biocompatibility of Nanoparticles: Effects of Carbon Nanofbers on Proliferation and Survival. -Jpn. J. Protozool. 40 (2), 139–146.
8	8. Vinokhodov D. O. Biotesting on Infusoria Cultures in Diagnostic Prevention of Animals Food Poisonings (review). - Veterinary Patologiya. 2006.1, 90-96.
9	9. Shadrin I. A., Vasilyeva T. V. Assessment of Toxicity of Soils of Krasnoyarsk Vicinities by Biotesting Methods. - Bulletin of the Krasnoyarsk State Agricultural University, 2015. 2, 32-38.
10	10. Ignatyev A. D., Isaev M. K., Dolgov V. A., etc. Modifcation of a Method of Biological Assessment of Foodstuff by Means of Ciliate of TetrakhimenPiriphormis. - Food Questions, 1980. - 1, 70-71.
11	11. Demidenko G. A., Shuranov V. V. The Assessment of Toxicity of Forages with Use of Infusoria of Paramecium Caudatum. - Bulletin of the Krasnoyarsk State Agricultural University. 2015, 10, 5-11.
12	12. Recommendations about Assessment of Toxicity of a Surface Water of Landin the Conditions of Emergency Situations by Biotesting Express Method: Р 52.24.741. – Rostov-on-Don, 2010.
13	13. V. A. Senkevich (Burdova), T. G. Stojko, News of Higher Educational Institutions. Natural Sciences Series, Volga region, 2015. 6(3), 84-93.
14	14. Kokova V. E., Lisovsky G. M. Disproportionate and Flowing Culture of Protozoa. - Novosibirsk, Science, 1982
15	15. Nikitina L. I., Solodovnikova M. V. Inﬂuence of Iron Salt on Paramecium Caudatum Activity. - Topical Issues of Modern Science. 2009, 6-1, 53-59.
16	16. Kulikov Yu. I., Dadyan N. K., Cheremnykh E. G. Biological Assessment of Toxicity of Food Dyes. - Bulletin of the North Caucasian Federal University. 2009, 2, 63-65.
17	17. Grozdov A. O. Defnition of the General Toxicity on Paramecium Caudatum. - Compound Feeds. 2001, 4, 31-33.
18	18. Bubnov A.G., Buymova S.A., Gushchin A. A., etc. The Biotest Analysis – an Integrated Method of Assessment of Quality of Objects of the Environment. Ivanovo, Ivan. State Chemical - Technol. University. 2007.
19	19. Kokova V. E. Continuous Cultivation of Invertebrates. - Novosibirsk, Science, 1982.
20	20. Karpukhina O. V., Gumargaliyeva K.Z., Inozemtsev A. N., etc. Features of Kinetics of Growth of ParameciumCaudatum Culture in Model of an Oxidizing Stress. - Bulletin of the Kazan Technological University, 2015. 10 (18), 9-11.
21	21. Methodical Recommendations about Application of Methods of Biotesting for Assessment of Water Quality in Systems of Economic and Drinking Water Supply: MR TsOS PV P 005-95. - M., 1995.
22	22. Turovtsev V. D., Krasnov B.C. Bioindication. - Tver, Tver State University, 2004.pp. 153.
23	23. Ampere-second 1638157 USSR. The Way of Receiving Resinoid Microalgae / Bugorsky P. S., Rodov V.S., Semenov E. F., Klyachko-Gurvich G. L. (USSR). – The application 22.03.89 (the application No. 4665003 with priority date of the invention 22.03.1989). It is registered in the State register of inventions of the USSR 01.12.1990. Publ. 30.03.91, BI No. 12.
24	24. V.V. Polevoy, G.B. Maksimov (ed.), Methods of biochemical analysis of plants, Publishing house of the Leningrad University, Leningrad (1978), pp. 90-99.
25	25. A Study Guide on Hygienic Rationing of Medicines in Air of a Working Zone, Atmospheric Air of the Inhabited Places and Water Objects: MU 1.1.726-98. – M, 1998.
26	26. Plokhinsky N. A. Mathematical Methods in Biology. - Moscow, MGU Publishing House, 1978.