Ushbu tadqiqotda banan navlarini in vitro sharoitida mikroko‘paytirish protokollarini ishlab chiqish yoritilgan. Eksplantlar MS, va DKW ozuqa muhitlariga ekildi, ular o‘z navbatida o‘simlik o‘sish regulyatorlari, asosan BAP va IBA bilan boyitilgan edi. Natijalar shuni ko‘rsatdiki, banan eksplantlarining tirik qolishi va regeneratsiyasi sterilizatsiya sharoitlari hamda ozuqa muhitidagi gormonlar tarkibiga kuchli bog‘liq. Ayniqsa, 2.5–4.0 mg/L BAP va 0.1 mg/L IBA qo‘shil gan kuchaytirilgan MS muhiti nihollarni ko‘paytirish va ularning cho‘zilishida eng yaxshi natijalarni berdi.
Ushbu tadqiqotda banan navlarini in vitro sharoitida mikroko‘paytirish protokollarini ishlab chiqish yoritilgan. Eksplantlar MS, va DKW ozuqa muhitlariga ekildi, ular o‘z navbatida o‘simlik o‘sish regulyatorlari, asosan BAP va IBA bilan boyitilgan edi. Natijalar shuni ko‘rsatdiki, banan eksplantlarining tirik qolishi va regeneratsiyasi sterilizatsiya sharoitlari hamda ozuqa muhitidagi gormonlar tarkibiga kuchli bog‘liq. Ayniqsa, 2.5–4.0 mg/L BAP va 0.1 mg/L IBA qo‘shil gan kuchaytirilgan MS muhiti nihollarni ko‘paytirish va ularning cho‘zilishida eng yaxshi natijalarni berdi.
В данном исследовании освещена разработка протоколов микроклонального размножения сортов банана в условиях in vitro. Экспланты высевались на питательные среды MS и DKW, обогащённые регуляторами роста растений, в частности BAP и IBA. Результаты показали, что выживаемость и регенерация эксплантов банана находятся в сильной зависимости от условий стерилизации и гормонального состава питательной среды. Особенно хорошие результаты по размножению побегов и их удлинению были получены на обогащённой среде MS с добавлением 2.5–4.0 мг/л BAP и 0.1 мг/л IBA.
This study highlights the development of protocols for the in vitro micropropagation of banana varieties under laboratory conditions. Explants were cultured on MS and DKW nutrient media, which were supplemented with plant growth regulators, primarily BAP and IBA. The results showed that the survival and regeneration of banana explants were highly dependent on sterilization conditions and the hormonal composition of the nutrient media. In particular, enriched MS medium containing 2.5–4.0 mg/L BAP and 0.1 mg/L IBA provided the best results for shoot multiplication and elongation.
| № | Муаллифнинг исми | Лавозими | Ташкилот номи |
|---|---|---|---|
| 1 | Allayarov A.N. | “Biotexnologiya” laboratoriyasi mudiri, q.x.f.f.d., dotsent | Akademik M.Mirzayev nomidagi bog‘dorchilik, uzumchilik va vinochilik ilmiy-tadqiqot instituti |
| 2 | Bo'ronov F.Z. | kichik ilmiy xodim | Akademik M.Mirzayev nomidagi bog‘dorchilik, uzumchilik va vinochilik ilmiy-tadqiqot instituti |
| № | Ҳавола номи |
|---|---|
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