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Nucleic acids (DNA and RNA) are the most important biomolecules that play a key role in the transmission of hereditary information, protein biosynthesis and other biological processes. To carry out various studies such as sequencing, cloning and transfection, it is necessary to obtain pure nucleic acids. This article examined the literature data on the main methods for isolating nucleic acids, analyzed the advantages and disadvantages of methods for isolating nucleic acids, and also considered the prospects for the development of new methods for isolating nucleic acids.

  • O'qishlar soni 117
  • Nashr sanasi 26-03-2024
  • Asosiy tilIngliz
  • Sahifalar7-12
English

Nucleic acids (DNA and RNA) are the most important biomolecules that play a key role in the transmission of hereditary information, protein biosynthesis and other biological processes. To carry out various studies such as sequencing, cloning and transfection, it is necessary to obtain pure nucleic acids. This article examined the literature data on the main methods for isolating nucleic acids, analyzed the advantages and disadvantages of methods for isolating nucleic acids, and also considered the prospects for the development of new methods for isolating nucleic acids.

Havola nomi
1 Ali, N., Rampazzo, R. D. C. P., Costa, A. D. T., & Krieger, M. A. (2017). Current nucleic acid extraction methods and their implications to point-of-care diagnostics. BioMed research international, 2017. Hagarová, I. (2020). Magnetic solid phase extraction as a promising technique for fast separation of metallic nanoparticles and their ionic species: a review of recent advances. Journal of Analytical Methods in Chemistry, 2020. Ma, W., Li, X., Bai, Y., & Liu, H. (2018). Applications of metal-organic frameworks as advanced sorbents in biomacromolecules sample preparation. TrAC Trends in Analytical Chemistry, 109, 154-162. Rahman, M. M., & Elaissari, A. (2012). Nucleic acid sample preparation for in vitro molecular diagnosis: from conventional techniques to biotechnology. Drug discovRery today, 17(21-22), 1199-1207. Антонова, О. С., Корнева, Н. А., Белов, Ю. В., & Курочкин, В. Е. (2010). Эффективные методы выделения нуклеиновых кислот для проведения анализов в молекулярной биологии (обзор). Научное приборостроение, 20(1), 3-9. Аукенов, Н. Е., Масабаева, М. Р., & Хасанова, У. У. (2014). Выделение и очистка нуклеиновых кислот. Состояние проблемы на современном этапе. Наука и здравоохранение, (1), 51-53. Егунова, О. Р., Константинова, Т. А., & Штыков, С. Н. (2014). Магнитные наночастицы магнетита в разделении и концентрировании. Изв. Сарат. ун-та. Нов. сер. Сер. Химия. Биология. Экология, 14(4), 27-34. Yu, M. S. (2023). HYDROLYZATION FROM EGGSHELL MEMBRANE UNDER THE INFLUENCE OF PAPAIN ENZYME AND DETERMINATION OF TOTAL PROTEIN AMOUNT. Asian journal of pharmaceutical and biological research, 12(2). Менглиева, Ш. Ю., & Зоирова, Х. Т. ЯИЧНАЯ СКОРЛУПА КАК АЛЬТЕРНАТИВНЫЙ И ДЕШЁВЫЙ ОБЪЕКТ ДЛЯ ПОЛУЧЕНИЯ БИОЛОГИЧЕСКИ АКТИВНЫХ ДОБАВОК. Tursunova Solikha Zafar qizi. (2023). TO STUDY THE MEDICINAL PROPERTIES OF THE GINGER PLANT. Modern Scientific Research International Scientific Journal, 1(2), 134–144. https://doi.org/10.5281/zenodo.7932573 Mamatkulov Z.U., K. G. G. (2023). DETERMINATION OF THE CHEMICAL COMPOSITION OF THE PLANT CALENDULA OFFICINALIS. INTERNATIONAL BULLETIN OF MEDICAL SCIENCES AND CLINICAL RESEARCH, 3(9), 134–138. https://doi.org/10.5281/zenodo.8405927 https://biomolecula.ru/articles/metody-v-kartinkakh-ochistka-molekul-i-razdelenie-smesei https://www.helicon.ru/support/methodological/life-sciences/vydelenie-i-ochistka-nukleinovykh-kislot-kak-pravilno-vybrat-metod/
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