193

Bugungi kungacha fitoviruslarni diagnostika qilishda bir qator usullar qo‟llanilib kelingan bo‟lib, ularning har biri o‟ziga xos sezgirligi bilan bir-biridan farqlanadi. Bu usullar ichida immunologik usullar o‟zining sezgirligi va bir vaqtning o‟zida bir qancha namunani aniqlay olishi bilan afzalliklarga ega. So‟ngi yillarda biotexnologiyaning rivojlanishi natijasida paydo bo‟lgan immunologik usullar sezgirligining avvaldan qo‟llanilib kelingan usullardan sezgirligi jihatdan yuqori darajada turishi haqidagi ma‟lumotlar bir qator mualliflar tomonidan keltirib o‟tilgan. Shuning uchun ushbu ishda tomchi, ikkiyoqlama immunodiffuziya, immunoferment analizi (IFA) usulining «to‟g‟ri», «noto‟g‟ri» va «sendvich» kabi variantlarining sezgirlik darajasi solishtirilgan hamda ularning ichidan IFAning sendvich variantining sezgirlik darajasi eng yuqori, ya‟ni 10-9 ekanligi aniqlandi. Ushbu tajribalarda antigen sifatida kartoshka X-virusidan (KXV) foydalanildi.

  • Read count193
  • Date of publication21-03-2024
  • Main LanguageO'zbek
  • Pages966-973
Ўзбек

Bugungi kungacha fitoviruslarni diagnostika qilishda bir qator usullar qo‟llanilib kelingan bo‟lib, ularning har biri o‟ziga xos sezgirligi bilan bir-biridan farqlanadi. Bu usullar ichida immunologik usullar o‟zining sezgirligi va bir vaqtning o‟zida bir qancha namunani aniqlay olishi bilan afzalliklarga ega. So‟ngi yillarda biotexnologiyaning rivojlanishi natijasida paydo bo‟lgan immunologik usullar sezgirligining avvaldan qo‟llanilib kelingan usullardan sezgirligi jihatdan yuqori darajada turishi haqidagi ma‟lumotlar bir qator mualliflar tomonidan keltirib o‟tilgan. Shuning uchun ushbu ishda tomchi, ikkiyoqlama immunodiffuziya, immunoferment analizi (IFA) usulining «to‟g‟ri», «noto‟g‟ri» va «sendvich» kabi variantlarining sezgirlik darajasi solishtirilgan hamda ularning ichidan IFAning sendvich variantining sezgirlik darajasi eng yuqori, ya‟ni 10-9 ekanligi aniqlandi. Ushbu tajribalarda antigen sifatida kartoshka X-virusidan (KXV) foydalanildi.

№ Author name position Name of organisation
1 Fayziev V.. Biologiya kafedrasi mudiri Toshkent viloyati Chirchiq davlat pedagogika instituti
№ Name of reference
1 1. Arshad, J., Ahmad, N., Bushra, T., Muhammad, T., Abdul, M. (2012). Molecular characterization of capsid protein gene of potato virus X from Pakistan. African Journal of Biotechnology, Vol. 11(74), pp. 13854-13857
2 2. Abduraimova Kh.(2020). Effect of The Virus of The Yellow Dwarf Corn Mosaic Growth and Development of Varieties of Corn in Various Phases. Journal of Advanced Research in Dynamical and Control Systems, Vol . 6, Issue 12. pp. 602- 606
3 3. Ahmed, Sh., Ahmed, I., Yaqub, Т., Waseem, M., Tabassum, B., Masood, F., Khan, A. (2013). Molecular detection, phylogenetic analysis and designing of siRNA against Potato Virus X. Advance ments in Life Sciences, Vol . 1, Issue 1. pp. 37-44
4 4. Eshboev, F., Yusupova, E., Piyakina, G., Mejlumyan, L. (2019). Isolation and study of the amino acid composition of proteins from soybean for producing conjugates for elisa//Universum: Химия и биология: электрон. научн. журнал, №11(65), –c. 62-65.
5 5. Belinda, A., Roger, A., Jones, C. (2010). Genetic variability in the coat protein gene of Potato virus X and the current relationship between phylogenetic placement and resistance groupings. Arch Virol, 155:1349–1356
6 6. Ramazonov, B. R., Mutalov, K. A., Fayziev, V. B., & Koraev, S. B. (2020). Morphogenetic characteristics and biological activity of takyr and meadow soils of the republic of karakalpakstan (on the example of soils of chimbay district) morphogenetic characteristics and biological activity of takyr and meadow soils of the republic of karakalpakstan (on the example of soils of chimbay district). Journal Of Critical Reviews, 7(5), 243-249. DOI: http://dx.doi.org/10.31838/jcr.07.05.43
7 7. Fayziev, V., Javlieva, D., Kadirova, Z., Chirkov, S., Jurayeva, U., Vakhabov, A. (2020). Study of some biological properties necrotic isolate of potato virus X and phylogenetic analysis. International Journal of Psychosocial Rehabilitation, Vol. 24, Issue 09. – P. 455-465. ISSN: 1475-7192
Waiting