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Biochemical modification of secondary cellulose fabrics using various enzymes

Field of Science:Chemical EngineeringChemistry
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O_TJ.2024.02.16_bf416932.pdf

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To effectively utilize dual-layer cellulose, enzymes were employed. Cellulase, protease, lipase, and amylase enzymes were prepared for the biochemical modification of the material. Each sample of material after each digestion was incubated for 2 hours at 50°C in a drying oven, then subjected to modification. The effectiveness of the enzymes was evaluated based on the capillarity of the samples and the amount of glucose added to the digestion during material processing. The results showed that the capillarity of dual-layer cellulose after treatment with cellulase enzyme was 125 mm; the capillarity of single-layer cellulose with cellulase enzyme was 117 mm, with protease - 91 mm. Modification using a multifunctional complex was more effective, in particular, a composition consisting of amylase, lipase, cellulase, and protease yielded 107 mm; a composition of amylase, lipase, cellulase, and protease used in the usual mode - 110 mm, while the traditional method yielded 60 mm. The results of sugar determination slightly did not correspond to the capillarity results. This is because cellulase and protease enzymes do not participate in the breakdown of starch to glucose. After treating dual-layer cellulose with cellulase enzyme, the sugar content was 0.11 mg/ml; after treating single-layer cellulose with cellulase enzyme - 0.09 mg/ml, with protease - 0 mg/ml; a composition consisting of amylase, lipase, cellulase, and protease yielded 0.9 mg/ml; a composition of amylase, lipase, cellulase, and protease used in the usual mode yielded 1.1 mg/ml. The obtained results demonstrated a positive effect of the biochemical modification on the characteristics of dual-layer cellulose.

AUTHORS

G.Jo’rayeva

TOSHKENT TO'QIMACHILIK VA YENGIL SANOAT INSTITUTI

Tags

# protease# amylase# cellulase# lipase# primary and secondary cellulose tissue

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